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. 2012 Feb 20;590(Pt 8):1921–1941. doi: 10.1113/jphysiol.2011.222414

Figure 1. Degradation of eNAD in monkey whole muscle (WM) and circular muscle (CM) colon preparations.

Figure 1

A, original chromatograms of eNAD (0.2 μm) in the absence, (–) tissue, and presence, (+) tissue, of either WM or CM (30 s contact of substrate with tissue). The formation of eADPR and eADO was increased in the (+) tissue samples. No noticeable changes were observed in the peak of eNAD at 12.5 min, because this peak also contains eAMP formed from eNAD; LU, luminescence units. B, graphic representation of eADO formation in superfusate samples collected in the absence (–) or presence (+) of tissue. Note the increased formation of eADO in both WM and CM; the formation of eADO was greater in CM than WM. Asterisks denote significant differences from the amounts of eADO in (–) tissue samples (**P < 0.01, ***P < 0.001). There is a significant difference a significant increase in CM as compared to WM (†P < 0.05); number of experiments in parentheses. C, Western immunoblot analysis of CD38 showed no significant differences between the protein levels of CD38 in WM and CM. Density of each band is normalized to tubulin, which was used to control equal protein loading.