Figure 6. Spontaneous IJP and Vm. Spontaneous IJP recorded in wild type animals (A) were still recorded in the presence of l-NNA and drastically reduced by 1 μm MRS2500.
No spontaneous IJPs were recorded in P2Y1−/− mice (B) although the recording was ‘noisy’. To quantify spontaneous IJP the SD was calculated (C, right: comparison with previous drug addition, ***). Frequency distribution of the data from the recording illustrates in WT animals: (1) the presence of a tail towards the most negative values indicative of spontaneous IJP, (2) the shift to the right in the frequency distribution induced by l-NNA indicative of depolarization without a major effect on spontaneous IJP and (3) the absence of the tail after MRS2500 addition. In contrast, in P2Y1−/− mice the frequency distribution was symmetrical in control. l-NNA shifted the distribution to the right but MRS2500 did not change the frequency distribution.