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The predicted molecular weight of the LASV-NP-FLAG proteins and the estimated size of these proteins as detected by Western blot analysis (staining with α-FLAG antibody).
| Recombinant YF17D/LASV-NP viruses | Expected size (kDa) | Estimated observed size (kDa) |
|---|---|---|
| YF17D/LASV-VNP(1-876) | 40 | 39 |
| YF17D/LASV-NP(435-1296) | 36 | 36 |
| YF17D/LAS-NP(870-1707) | 36 | 36 |
| YF17D/LASV-NP(1-1296) | 53 | 53 |
| YF17D…3XFLAG-FMDV2A (empty vector) | 5 | 5.6 |
