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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Dev Growth Differ. 2013 Jan 28;55(2):282–300. doi: 10.1111/dgd.12035

Figure 3. Diagram of the regeneration experiment using the dn-fgfr1 transgenic zebrafish line.

Figure 3

Tg(hsp70l:dn-fgfr1-EGFP) fish were genotyped by outcrossing adult males to wild type females (A) and screening a subsample of embryos for EGFP expression after a 1-hour heat-shock treatment (B). Clutches of 50:50::hemizygous:wild type fish were reared for 3–6 months, after which the caudal fin and left maxillary barbel were removed (C); the right maxillary barbel was left intact as a control. After 24 hours at 28°C to allow for wound healing, fish were haphazardly split between heat-shock tanks (HS = >37°C for one hour/day) and no heat-shock tanks (NHS = constant 28°C; D). Barbels and caudal fins were allowed to regenerate for 14 days, then collected for analysis (E).