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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Dev Growth Differ. 2013 Jan 28;55(2):282–300. doi: 10.1111/dgd.12035

Table 1. Primers for reverse transcription polymerase chain reaction (RT-PCR) detection of FGF receptors, ligands and downstream targets.

Product sizes and specificity were predicted using NCBI’s Primer BLAST on zebrafish RefSeq RNA.

Gene ZBD-GENE Primer Sequence 5’-3’ predicted size (bp)
fgf20a 060110-1 fgf20a-F1
fgf20a-R1
GCAGATTTGGTATATTGGAATTCAT
CTAGAACATCCTTGTAAAGCTCAGG (Stoick-Cooper et al., 2007)
348
fgf24 030708-1 fgf24-F1
fgf24-R1
GCAAGAAGATTAACGCCAATGG
TTTAGGTCGACCCTTTCG
272
fgfr1a 980526-255 fgfr1a-F1
fgfr1a-R1
AAACCGTGAAGTTTCGTTGC
TCACCTCGATGTGTTTCAGC
372
fgfr1b 060503-14 fgfr1b-F1
fgfr1b-R1
AACGTGTCCTTTGAGGATGC
CTGGAGGAGAGACGTGAAGG
365
fgfr2 030323-1 fgfr2-F1
fgfr2-R1
TTGGCGGGTAACTCTATTGG
TCAGGGAGGTCGTATTCTGG
371
fgfr3 000816-1 fgfr3-F1
fgfr3-R1
ACTGGACAGAGTCGGACAGC
CAGACATCCTCAACCGTTCC
321
fgfr4 980526-488 fgfr4-F1
fgfr4-R1
GCTTACCAAGTGGCAAGAGG
TGCAGTTGGAGGGTTTATCC
367
il17rd 020320-5 il17rd-F3
il17rd-R3
CAGAGATTCGGGGATTTACG
TGGCGCTGCGAGTGGAGCTG
302
pea3 990415-71 pea3-F1
pea3-R1
GCCTGGCTGCCCATCCATGT
GCAATAGGTGCAGCGGGTGGT
674