Table 1. Primers for reverse transcription polymerase chain reaction (RT-PCR) detection of FGF receptors, ligands and downstream targets.
Product sizes and specificity were predicted using NCBI’s Primer BLAST on zebrafish RefSeq RNA.
| Gene | ZBD-GENE | Primer | Sequence 5’-3’ | predicted size (bp) | |
|---|---|---|---|---|---|
| fgf20a | 060110-1 | fgf20a-F1 fgf20a-R1 |
GCAGATTTGGTATATTGGAATTCAT CTAGAACATCCTTGTAAAGCTCAGG (Stoick-Cooper et al., 2007) |
348 | |
| fgf24 | 030708-1 | fgf24-F1 fgf24-R1 |
GCAAGAAGATTAACGCCAATGG TTTAGGTCGACCCTTTCG |
272 | |
| fgfr1a | 980526-255 | fgfr1a-F1 fgfr1a-R1 |
AAACCGTGAAGTTTCGTTGC TCACCTCGATGTGTTTCAGC |
372 | |
| fgfr1b | 060503-14 | fgfr1b-F1 fgfr1b-R1 |
AACGTGTCCTTTGAGGATGC CTGGAGGAGAGACGTGAAGG |
365 | |
| fgfr2 | 030323-1 | fgfr2-F1 fgfr2-R1 |
TTGGCGGGTAACTCTATTGG TCAGGGAGGTCGTATTCTGG |
371 | |
| fgfr3 | 000816-1 | fgfr3-F1 fgfr3-R1 |
ACTGGACAGAGTCGGACAGC CAGACATCCTCAACCGTTCC |
321 | |
| fgfr4 | 980526-488 | fgfr4-F1 fgfr4-R1 |
GCTTACCAAGTGGCAAGAGG TGCAGTTGGAGGGTTTATCC |
367 | |
| il17rd | 020320-5 | il17rd-F3 il17rd-R3 |
CAGAGATTCGGGGATTTACG TGGCGCTGCGAGTGGAGCTG |
302 | |
| pea3 | 990415-71 | pea3-F1 pea3-R1 |
GCCTGGCTGCCCATCCATGT GCAATAGGTGCAGCGGGTGGT |
674 | |