Figure 1.
MMP-2 cleavage of PEG controlled cellular uptake of quantum dots. Streptavidin-coated quantum dots (QD-strep) (red – top row) achieved little internalization by GFP-expressing MDA-MB-435 melanoma cells (green – bottom row, merged with top row). Functionalization of the quantum dot surface with biotin-CPP increased cellular uptake, while addition of removable PEG (biotin-substrate-PEG) decreased the internalization by blocking interactions of the CPP with the cell surface. Cellular uptake was recovered in the presence of MMP-2 with cleavage of the substrate-PEG linker, as the CPPs could interact with the cell surface following the removal of PEG. Reprinted with permission from Ref 63 Copyright 2009 American Chemical Society.