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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012 Sep 13;5(1):31–48. doi: 10.1002/wnan.1197

Figure 6.

Figure 6

Cellular uptake of CPP-functionalized HPMA was controlled by the dissociation of neutralizing inhibitory domains in response to UV illumination. Charge-neutralizing groups conjugated to the CPP by UV-labile linkers prevented internalization of FITC labeled polymer (green) in PC-3 prostate cancer cells kept in the dark (bottom). Exposure to UV light for 10 minutes was sufficient to remove the neutralizing groups and allow significant increase in cellular uptake (top). Lysosomes were labeled with LysoTracker Red and colocalized, in part, with the internalized HPMA. The delivery to lysosomes provided the acidic conditions necessary to allow intracellular drug release from the carrier using a pH-sensitive hydrazine linker. Reprinted from Ref 84. Copyright 2011 with permission from Elsevier.