Figure 2.

Cell cycle analysis of AML cell lines following treatment with LeTx. Control cells are represented in the left panels, and cells treated with 10 nM LeTx for 48 hours are represented in the right panels. Cells are gated on width versus forward scatter (R1). Cells in G₀/G₁ are gated M2, those in G₂/M are gated M3, and those in pre-G₀/G₁ (dead) are gated M4 or M5. One of the cell lines that did not show a cytotoxic response to LeTx, ML1 (A), did show cell cycle arrest, whereas the other, U937 (B), did not. Similarly, one of the LeTxsensitive cell lines ML2 (C) showed cell cycle arrest in addition to cell death, whereas the other, TF1-vRaf (D), showed complete cell death following treatment.