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. 2013 Feb 1;6(1):25–32. doi: 10.1593/tlo.12313

Figure 4.

Figure 4

Single-cell intracellular staining of phospho-ERK1/2 in four AML cell lines using flow cytometry. TF1-vRaf (A) and ML-2 (B) cells, which showed a cytotoxic response to the LF-mediated inhibition of the MAPK pathway, were positive for the presence of phospho-ERK1/2 as evidenced by an RFI > 2 between cells stained for phospho-ERK1/2 (gray) and cells incubated with an isotype control (black). Cells are gated on width versus forward scatter (R2 and R3). ML-1 (C) and U937 (D) cells, which were resistant to the cytotoxicity of LeTx, were negative for phospho-ERK1/2 with RFI < 1.5.