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Analysis of the mechanism of LeTx-mediated cytotoxicity in TF1-vRaf cells using annexin V/PI (A) and active caspase staining (B). TF1-vRaf cells incubated with 10 nM LeTx for 24 hours (right panel) stained positively with both annexin V (FL1-H) and PI (FL2-H). Incubation of LeTx-treated TF1-vRaf cells with a cell permeable, FITC-conjugated active caspase inhibitor revealed the absence of active caspases following incubation with LeTx (gray).
