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(A) Competition assay to characterize specificity of uptake of propargyl choline in cells. HeLa cells were co-incubated with a fixed concentration (2 mM) of propargyl choline and increasing concentration of choline chlorine (0–60 mM). After incubation, the cells were fixed and stained with Alexa 488 azide. (B) Decrease in MFI (corresponding to uptake of propargyl choline) as a function of increasing concentration of choline chloride in cell culture media.
