Figure 1.

Secretion of tumor regulatory molecules by irradiated and non-irradiated CAFs. Quantitative determinations of growth factors and cytokines in culture supernatants from five different donors were measured by multiplex protein arrays. Only factors giving values above the detection limit of the assay are represented. Panel (A) demonstrates expression of inflammatory molecules. The following cytokines were included in the array: IL-1β, IL-6, IL-8, and TNF-α. Panel (B) shows array of angiogenic factors including SDF-1α, angiogenin, angiopoietin, TSP-2, and VEGF. Finally, (C) presents array of detectable tumor growth regulators, comprising HGF, bFGF, and PDGF. Mean values from five different donor samples are shown; statistical analyses were performed by applying paired samples Student's t test. Statistically significant differences are indicated by asterisk (P < .05).