Figure 3.

Effect of CAF-CM on the proliferative and migratory capacity of HUVECs. CAFs were treated with 1x 18 Gy and incubation medium was conditioned in serum-free conditions for 4 to 6 days post-irradiation. Proliferative capacity of HUVECs was monitored on E-plates (A) and migratory capacity in double-chambered CIM plates (B), with readout by the xCELLigence System for both types of plates. For both experiments, HUVECs were exposed to (1:1) non-irradiated CAF-CM (red line), and (1:1) 1x 18 Gy irradiated CAF-CM (green line) from one randomly selected donor. Similar profiles were obtained after running the assay with CAF-CM from seven different donors. Columns in (C) represent mean and SEs of CI values after a 20-hour observation of HUVEC migratory behavior, averaged from seven independent experiments using CAF-CM from seven randomly selected donors. Error bars represent SD.