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. 2013 Feb 1;6(1):66–74. doi: 10.1593/tlo.12349

Figure 4.

Figure 4

Effect of CAF-CM on endothelial cell tube formation. CAFs were treated with 1x 18 Gy and incubation medium was conditioned in serum-free conditions between days 4 and 6 post-irradiation. HUVECs were plated in Matrigel-coated multiwell plates, and after an attachment phase of 2 hours, HUVECs were exposed to CM from irradiated (18 Gy) and non-irradiated CAFs (0 Gy) and incubated for another 20 hours. Images show tube formation by HUVECs after 20 hours of growth in Matrigel. In (A), tube formation by HUVECs exposed to standard (cell-free) incubation medium is shown, whereas (B) and (C) demonstrate tube-forming HUVECs exposed to CM from non-irradiated CAFs (CAF-CM 0 Gy) and irradiated CAFs (CAF-CM 18 Gy), respectively. Bars, 320 µm. Panel (D) reflects quantification of HUVEC forming tubes/well. Data represent average values from three independent experiments (error bars represent SD).