FIG 1 .

CFT073 rpoS has a colonization defect at 48 hpi in CBA/J murine model UTI. (A) CFT073 rpoS and CFT073 lacZYA were coinoculated at a 1:1 ratio into CBA/J mice (n = 17). A functional copy of rpoS was reintroduced into CFT073 rpoS via generalized transduction with ϕEB49. This transductant (rpoS::ϕrpoS) was also coinoculated with the wild type (wt) into CBA/J mice (n = 8). Mice were sacrificed at 48 hpi. Bacteria from bladder homogenates were enumerated on MacConkey’s medium plus lactose. Lines are drawn at the geometric mean relative competitive index (RCI). Statistical significance was assessed by a Mann-Whitney U test relative to a hypothetical RCI of 1. (B) Manipulations were carried out as described for panel A except that single strains were used (wt, n = 22; rpoS, n = 24). Lines are drawn at the geometric mean CFU/bladder. Total burdens of animals coinfected with the CFT073 wild-type and rpoS strains at 48 hpi (from panel A) are included for comparison. Statistical significance was assessed by the Mann-Whitney U test.