FIG 3 .

Levels of peroxide in urine are elevated in urine from infected CBA/J mice. CBA/J mice were infected with either CFT073 or CFT073 rpoS (n = 12 each). At 24 and 48 hpi, urine was collected from these animals and H₂O₂ concentrations were measured using a Xylenol Orange-based quantitative peroxide assay kit (Pierce). Data are represented as mean fold change in [H₂O₂] ± SEM relative to PBS mock-infected control animals. Mean fold changes in [H₂O₂] did not differ between mice infected with CFT073 and mice infected with CFT073 rpoS at 24 or 48 hpi, but there was a significant change in [H₂O₂] in infected animals from 24 to 48 hpi, as assessed by an unpaired t test and a paired t test, respectively.