TABLE 2 .

Summary of NTML and extra Tn insertion mutants in the S. aureus USA300 LAC JE2 genome, based on mapping software results using the USA300 FPR3757 GenBank genome

Type of insertion	NTML clones		Extra insertion mutants
	Count	%	Count	%
Transposon insertions between and within genes
All insertions	1,952	100	16,845	100
Insertions between genes	0	0	4,632	27
Insertions affecting ≥1 genea	1,956	100	12,228	73
Insertions affecting 2 genesa	4		15
Insertions affecting >2 genesa	0		0
Transposon insertions within CDS genes
All insertions	1,954	100	12,034	100
Within the first third of gene length	1,336	68	3,889	32
Within the second third of gene length	472	24	3,934	33
Within the last third of gene length	146	7	4,211	35
Distinct CDS genes	2,560	100	2,560	100
Distinct CDS with 0 insertions	615	24	661	26
Distinct CDS with ≥1 insertion	1,945	76	1,899	74
Within the first third of gene length	1,332		1,194
Within the second third of gene length	472		1,336
Within the last third of gene length	146		1,456

^aGene = CDS, rRNA, tRNA, pseudogene, or other gene.