Figure 2. Structure of A. aeolicus TatC.

a, Cartoon representation of AaTatC coloured from blue at the N-terminus to red at the C-terminus. The position within the membrane bilayer is predicted by MD simulations (Supplementary Fig. 5). A semi-ordered molecule of LMNG detergent is present and is shown in purple. b, Surface representation of the AaTatC structure. The left hand views in a and b are the same orientation. c, Positions of inactivating substitutions in EcTatC identified by genetic screening³² (green), targeted substitution^17,34 (orange), or both (yellow), mapped to AaTatC (full details in Supplementary Table 2). d, Sequence conservation at the surface of TatC displayed in the Consurf⁴⁶ colouring scheme in which maroon represents high, and cyan low, sequence conservation. c, d, Dotted regions labelled (1) and (2) highlight conserved functional regions of the structure.