Figure 3.

Contact hypersensitivity to DNFB is inhibited by pretreatment of skin with antibodies to heat shock proteins 27 (HSP27) and 70 (HSP70). (A) C3H/HeN mice were treated with anti-HSP antibodies (2µg/mouse in 100 µl of PBS). After 2h, the mice were sensitized with DNFB on the antibody treated site. The mice were ear challenged 5 days later and auricular thickness was measured at 24 hours. The application of anti-HSP antibodies significantly inhibited the CHS response (*p<0.05). Results were expressed as change in auricular thickness ± SEM. There were four mice per group and each experiment was repeated twice with the same results. The results are from one representative experiment. The group difference was compared using ANOVA test followed by Tukey’s post hoc test for multiple comparison adjustment. B) Histologic sections were taken from representative mice at 24 hours after DNFB challenge, stained with hematoxylin and eosin, and photomicrographs were taken (original magnification ×10 for all sections).