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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):675–683. doi: 10.4049/jimmunol.182.1.675

Figure 4.

Figure 4

Effect of HSP27 and −70 on cytokines by draining lymph node cells after DNFB sensitization in C3H/HeN mice. C3H/HeN mice were pretreated with anti-HSP27 and anti-HSP70 or species matched control antibodies and sensitized with 25µl of 0.5% DNFB. Primed lymph node cells were isolated after 5 days from the draining lymph nodes of DNFB sensitized mice and placed in cultures with DNBS labeled BMDC. Treatment of BMDC with anti-HSP27/70 antibody significantly inhibited the production of IL-17 and IFN-γ compared to untreated BMDC (*p<0.05). There was a corresponding increase in IL-4 and IL-10 production under the same conditions. The results are the mean ± SD with 3 mice per group and each experiment was repeated twice with identical results. The data shown are from one representative experiment.