Figure 8.

Inhibition of DNFB contact hypersensitivity by anti-HSP antibodies can be adoptively transferred by splenocytes. C3H/HeN mice were treated with either a combination of anti-HSP27 and anti-HSP70 or control antibody and the site of antibody application was then treated with 0.5% DNFB. Five days later 50×10⁶ splenocytes from these mice were transferred into naïve syngeneic recipients. The recipients were then sensitized and ear challenged with DNFB. The adoptive transfer of splenocytes from HSP treated donor mice led to significant inhibition (**p<0.001) of contact hypersensitivity in the recipient naïve mice compared to the control recipient mice. Results were expressed as change in auricular thickness ± SEM. There were four mice per group and each experiment was repeated twice with identical results. The data shown are from one representative experiment.