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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):675–683. doi: 10.4049/jimmunol.182.1.675

Figure 9.

Figure 9

Production of proinflammatory cytokines by HSP27 stimulated bone marrow derived dendritic cells (BMDCs) is dependant on TLR4. BMDCs from C3H/HeN and C3H/HeJ mice were treated with recombinant mouse HSP27. Polymyxin B (10µg/ml) was added to the culture to rule out any LPS contamination. There was a significant increase in IL-12p35, TNF-α, IL-1β (*p<0.05) and IL-12p40, IL-6 (**p<0.001) in C3H/HeN mice compared to C3H/HeJ mice. C3H/HeJ mice, on the other hand, showed an increase in IL-23p19 (*p<0.05) on stimulation with HSP27. There were two mice per group and each experiment was repeated twice with identical results. The data shown are from one representative experiment.