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. 2012 Jan 10;29(4):530–540. doi: 10.3892/ijmm.2012.883

Figure 6.

Figure 6

Effects of IL-33 silencing on the mRNA and protein expression of pro-inflammatory and pro-destructive mediators in TNF-α stimulated RA-SFs. RA-SFs (n=5) were preincubated for 24 h with 10 nM control siRNA or specific IL-33 siRNA followed by TNF-α stimulation (10 ng/ml) for 24 h. mRNA and protein expression of (A) the pro-inflammatory mediators IL-6, IL-8 and MCP-1, and (B) the pro-destructive mediators MMP-1, MMP-3, and TIMP-1 were analyzed by real-time PCR or ELISA. +P≤0.05 Mann-Whitney U-test vs. control siRNA. Bars indicate means ± SEM.