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Chronological change of the number of MGFs isolated from 10-, 30- and 52-week-old mice. The cell growth curve of MGFs at each culture day was different at each age. Significant differences in the cell number at day 8 of culturing were detected between 10- and 30-week-old, and 30- and 52-week-old mice (*P<0.05). A significant difference was also observed between 10- and 52-week-old mice (**P<0.01). The cell growth curves of the MGFs are different in the 10-, 30- and 52-week-old mice.
