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. 2013 Feb 15;7:11. doi: 10.3389/fncel.2013.00011

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Copyright © 2013 Yoshikawa, Samata, Ogura, Miyamoto and Takahashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Analyses of iPSC-derived midbrain DA neurons in animals treated with VPA, ZNS, or E2. (A) Representative immunohistologic images of grafts (M2M6; green) containing DA neurons (TH; red), FOXA2 (ventral-midbrain marker; red), and TH (DA neuron marker; green). The scale bar applies to all pictures and represents 500 μm (upper) and 50 μm (lower). A comparison between each group for (B) average graft volume, (C) the number of TH⁺ cells per graft, and (D) the number of TH⁺FOXA2⁺ cells (midbrain DA neurons) per graft. The data are presented as the mean ± SD. (^*P < 0.05, ^**P < 0.01; n = 8 control, n = 6 VPA, ZNS, E2).