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. 2012 Sep 6;30(5):1041–1047. doi: 10.3892/ijmm.2012.1121

Figure 1.

Figure 1.

HCV genome and recombinant proteins. (A) The HCV genome contains a single major open reading frame (ORF) flanked by untranslated regions (UTRs). The 10 proteins encoded within the main ORF are indicated by alternated shading. (B) Genomic fragments of HCV were cloned into the prokaryotic expression vectors, pET32a(+), pQE30, or pGEX-4T-2, in-frame downstream of the 6-His-tag or glutathione S-transferase (GST)-tag coding sequence.