Figure 2.

(A) Schematic diagram of a colloidal gold-based DAS-LFIA strip. The strip is composed of a sample pad, a conjugate pad that contains the core combined with NS3 (1183–1947 aa) for double antigen sandwich LFIA and antibody for indirect LFIA, and an absorbent pad attached to a nitrocellulose membrane, with a test line and an IgG control line impregnated onto the surface of the membrane. (B) Representative results of LFIA with serum. T, test line is present for samples with HCV antibody and absent for samples without HCV antibody; C, control line shows a normal flow of the liquid through the strip. Two lines indicate a positive result and one line indicates a negative result.