Table I.

Sequences of oligonucleotide primers that were used to validate the expression of several different HCV segment genes.

No.	Primer sequences	Primary description	Enzyme site
F1	5′-CGGGATCCATGAGCACGAATCCTAAACC-3′	Core-190	BamHI
R1	5′-TTAAGCTTCTGAAGCGGGCACAGTC-3′		HindIII
F2	5′-CGGGTACCATGAGCACGAATCCTAAAC-3′	Core-68	KpnI
R2	5′-TTGGATCCACGTGCCTTGGGGATA-3′		BamHI
F3	5′-CGGGTACCACGCAAGACTGCAATTGTT-3′	E1 300–407	KpnI
R3	5′-TTAAGCTTGCTTGGCGCCTGGT-3′		HindIII
F4	5′-CGGGATCCTACCAAGTGCGCAATTC-3′	E1 192–318	BamHI
R4	5′-CGAAGCTTATGCCATGCGATGACC-3′		HindIII
F5	5′-AAGGATCCACACCAGGCGCCAAG-3′	E2 403–642	BamHI
R5	5′-TTGAATTCCGCTTCCAGCCTGTG-3′		EcoRI
F6	5′-CCGGTACCTTGGAGAACCTCGTAAT-3′	P7 747–807	KpnI
R6	5′-TGGAATTCGTATGCCCGCTGAG-3′		EcoRI
F7	5′-TTGGTACCTGTGGCGGCGTTGTT-3′	NS2 817–1022	KpnI
R7	5′-CGGAATTCCCACCCCTTGGAGACCAT-3′		EcoRI
F8	5′-TTGGTACCCAGACGAGAGGCCTCCTAG-3′	NS3 1034–1193	KpnI
R8	5′-TTGGATCCGTCCACCGCCTTAGCC-3′		BamHI
F9	5′-AAGGATCCGTGTGCACCCGTGGAGT-3′	NS3 1183–1476	BamHI
R9	5′-GGAAGCTTGGAGCGTGGTTGTCTCAAT-3′		HindIII
F10	5′-CCGAATTCTTCAGCCTTGACCCTAC-3′	NS3 1463–1656	EcoRI
R10	5′-TTAAGCTTGCGTGACGACCTCC-3′		HindIII
F11	5′-TTGGTACCGTCCTGGCTGCTCTG-3′	NS4A 1665–1710	KpnI
R11	5′-CGAAGCTTGGCACTCTTCCATCTCA-3′		HindIII
F12	5′-TTGGTACCCCTGGAGCCCTTGTAGT-3′	NS4B 1888–1937	KpnI
R12	5′-TTGAATTCGCTCTCCGGCACGTAG-3′		EcoRI
F13	5′-AAGGTACCGCAGAGGAGGATGAGC-3′	NS5A 2258–2419	KpnI
R13	5′-CGGAATTCGCAGCACACGACATCTT-3′		EcoRI
F14	5′-TTGGTACCTGGACAGGCGCACTCGT-3′	NS5B 2425–2733	KpnI
R14	5′-TTGTCGACCGAGCATGGTGCAGTCC-3′		SalI

F, forward primer; R, reverse primer. HCV, hepatitis C virus. The underlined sequences show the base sequences of the enzyme sites.