Table I.
No. | Primer sequences | Primary description | Enzyme site |
---|---|---|---|
F1 | 5′-CGGGATCCATGAGCACGAATCCTAAACC-3′ | Core-190 | BamHI |
R1 | 5′-TTAAGCTTCTGAAGCGGGCACAGTC-3′ | HindIII | |
F2 | 5′-CGGGTACCATGAGCACGAATCCTAAAC-3′ | Core-68 | KpnI |
R2 | 5′-TTGGATCCACGTGCCTTGGGGATA-3′ | BamHI | |
F3 | 5′-CGGGTACCACGCAAGACTGCAATTGTT-3′ | E1 300–407 | KpnI |
R3 | 5′-TTAAGCTTGCTTGGCGCCTGGT-3′ | HindIII | |
F4 | 5′-CGGGATCCTACCAAGTGCGCAATTC-3′ | E1 192–318 | BamHI |
R4 | 5′-CGAAGCTTATGCCATGCGATGACC-3′ | HindIII | |
F5 | 5′-AAGGATCCACACCAGGCGCCAAG-3′ | E2 403–642 | BamHI |
R5 | 5′-TTGAATTCCGCTTCCAGCCTGTG-3′ | EcoRI | |
F6 | 5′-CCGGTACCTTGGAGAACCTCGTAAT-3′ | P7 747–807 | KpnI |
R6 | 5′-TGGAATTCGTATGCCCGCTGAG-3′ | EcoRI | |
F7 | 5′-TTGGTACCTGTGGCGGCGTTGTT-3′ | NS2 817–1022 | KpnI |
R7 | 5′-CGGAATTCCCACCCCTTGGAGACCAT-3′ | EcoRI | |
F8 | 5′-TTGGTACCCAGACGAGAGGCCTCCTAG-3′ | NS3 1034–1193 | KpnI |
R8 | 5′-TTGGATCCGTCCACCGCCTTAGCC-3′ | BamHI | |
F9 | 5′-AAGGATCCGTGTGCACCCGTGGAGT-3′ | NS3 1183–1476 | BamHI |
R9 | 5′-GGAAGCTTGGAGCGTGGTTGTCTCAAT-3′ | HindIII | |
F10 | 5′-CCGAATTCTTCAGCCTTGACCCTAC-3′ | NS3 1463–1656 | EcoRI |
R10 | 5′-TTAAGCTTGCGTGACGACCTCC-3′ | HindIII | |
F11 | 5′-TTGGTACCGTCCTGGCTGCTCTG-3′ | NS4A 1665–1710 | KpnI |
R11 | 5′-CGAAGCTTGGCACTCTTCCATCTCA-3′ | HindIII | |
F12 | 5′-TTGGTACCCCTGGAGCCCTTGTAGT-3′ | NS4B 1888–1937 | KpnI |
R12 | 5′-TTGAATTCGCTCTCCGGCACGTAG-3′ | EcoRI | |
F13 | 5′-AAGGTACCGCAGAGGAGGATGAGC-3′ | NS5A 2258–2419 | KpnI |
R13 | 5′-CGGAATTCGCAGCACACGACATCTT-3′ | EcoRI | |
F14 | 5′-TTGGTACCTGGACAGGCGCACTCGT-3′ | NS5B 2425–2733 | KpnI |
R14 | 5′-TTGTCGACCGAGCATGGTGCAGTCC-3′ | SalI |
F, forward primer; R, reverse primer. HCV, hepatitis C virus. The underlined sequences show the base sequences of the enzyme sites.