Figure 3.

Effects of gastrodin treatment on the regulation of smooth muscle gene expression. (A) VSMCs were preincubated for 2 h with gastrodin (200 μg/ml) and then stimulated with PDGF (20 ng/ml) for 48 h. The protein levels of SMA, SM22α, smoothelin and desmin were determined by western blot analysis and quantified by densitometry. (B) Bar graphs showing the quantification of the western blot analysis results. The results are expressed as a percentage of the controls (^*P<0.05 vs. control group; ^#P<0.05 vs. PDGF alone).