Figure 1.

Expression of TRPC3 in MCF-7 cells. (A) The expression of TRPC channels in MCF-7 cells was examined by RT-PCR. The PCR reaction yielded RNA transcript, of expected size, for TRPC3 but not TRPC1, 4 or 5. GAPD positive controls were found in each sample. (B) Immunostaining of TRPC3 in MCF-7 breast cancer cells. TRPC3 expression was seen throughout the cell cytoplasm and cell membrane. Arrows indicate location of antibody in cell membrane. Inset at the top right corner shows peptide negative control (26). Scale bar, 40 μm. (C) Western blot analyses showing the presence of TRPC3 protein in MCF-7 breast cancer cells.