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. 2012 Jan 11;29(4):607–612. doi: 10.3892/ijmm.2012.884

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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The effect of alpinetin on the protein expression of Bcl-2 family members and XIAP and on the release of mitochondrial cytochrome c. BxPC-3 cells were treated with different concentrations (0, 20, 40, 60 and 80 μg/ml) of Alpinetin for 48 h. (A) The expression of Bcl-2, Bcl-xL, Bax and XIAP were determined by western blot analysis. (C) The expression of cytochrome c in mitochondria and cytoplasm were also examined by western blot analysis. (B and D) The western blot results were further analyzed using Gel-Pro Analyzer 4.0 software (^*P<0.05). Results are representative of three independent experiments.