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. 2012 Jun 14;30(3):473–479. doi: 10.3892/ijmm.2012.1029

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 3. — Evaluation of hepatic stellate cell (HSC) activation. (A) Immunohistochemical evaluation showed increased α-SMA positive cells in the liver of OLETF/MCD. The number decreased in OLETF/MCD+silymarin rats. Data are presented as the number of α-SMA positive cells in 30 40x fields (1.3 mm², approximately 3,000 hepatocytes). (B) Analysis of α-SMA mRNA expression in the liver; the results are compatible to these of the immunohistochemical study. (C) Analysis of α-SMA mRNA expression in HSCs, isolated from the experimental animals; the results are in accordance with those of the whole liver study. ^*P<0.05 compared to LETO/vehicle. ^**P<0.05 compared to OLETF/MCD.