Table I.
Primers for qRT-PCR and sequences of miR-1187 mimic and non-specific mimic.
| Name | Sequence (5′-3′) |
|---|---|
| miR-1187 | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTACAC |
| F: CCCGGCTATGTGTGTGTGTATGT | |
| R: GTGCAGGGTCCGAGGT | |
| miR-1187 mimic | F: UAUGUGUGUGUGUAUGUGUGUAA |
| R: AUACACACACACAUACACACAUU | |
| Non-specific mimic | F: UCACAACCUCCUAGAAAGAGUAGA |
| R: AGUGUUGGAGGAUCUUUCUCAUCU | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT | |
| Caspase-8 | F: TGCCCTCAAGTTCCTGTGCTTGGAC |
| R: GGATGCTAAGAATGTCATCTCC | |
| β-actin | F: CTAGGCACCAGGGTGTGAT |
| R: TGCCAGATCTTCTCCATGTC |
RT, miRNA special reverse transcription primer; F, forward primer; R, reverse primer.