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LMP1 upregulates survivin expression and its Thr34 phosphorylation by p53 (A and B) in CNE1 and NP69 cells expressing LMP1 and (C and D) using siRNA targeting p53. α-tubulin was used as an internal control. Cells transfected with siRNA targeting p53 were incubated for 72 h. Expression of p53, phosphorylated p53 (Ser20), survivin, and phosphorylated survivin (Thr34) was detected. Untransfected cells (untreated) and cells transfected with pSUPER constructs were used as controls.
