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. 2012 Jan 17;29(4):574–580. doi: 10.3892/ijmm.2012.889

Figure 5.

Figure 5

Apoptosis and cell cycle analysis were examined by FCM and western blot analysis. (A) FCM results show that the apoptosis rate is less changed after p53 gene knockdown in CNE1-LMP1 cells, with P>0.05 by the Student’s t-test, compared with the controls. (B) Western blot analysis shows that the caspase-3 protein level does not change in CNE1- LMP1 cells transfected with p53-siRNA. (C) FCM results show that targeting p53 by siRNA could increase the number of CNE1-LMP1 cells in the S phase (*P<0.05), but decrease those in the G0/G1 phase (#P<0.05). The number of cells in the G2/M phase remained unchanged in p53siRNA transfected cells. (D) Downregulated G1/S phase checkpoint-related proteins, CDK2, CDK4 and cyclin D1, were observed in CNE-LMP1 cells transfected with p53siRNA.