Figure 7. RSKs are required for the increase in size induced by ET-1.

(A) Representative images of cardiomyocytes that were unstimulated (i, Control), or exposed (24 h) to PD184352 (ii), BI-D1870 (iii), ET-1 (iv), or ET-1 plus PD184352 (v) or BI-D1870 (vi). Cells were immunostained for troponin T. The experiment was performed four times with similar results. Scale bar=100 μm. (B) Cardiomyocyte surface area was measured for each condition, the average values being taken from each experiment as a single observation. Results are means±S.E.M. (n=4). *P<0.001 relative to the control, **P<0.001 relative to ET-1 and #P<0.01 relative to ET-1.