Table 4.
Effect of bichalcophenes, benzo[a]pyrene (B[a]P), and S9 mix on Salmonella typhimurium TA1535 viability as assessed by colony formation on plate
| Compound |
Viability at B[a]P and bichalcophene concentrationsa (colonies/plate (% of control))
|
|
|---|---|---|
| 5/10 μM | 10/20 μM | |
| None | 63.0 ± 5.9 (100) | |
| B[a]P (20 μM) | 67.9 ± 4.7 (108) | |
| 1A | 62.3 ± 4.1 (99) | 64.7 ± 5.1 (103) |
| 1B | 63.0 ± 5.9 (100) | 64.3 ± 3.7 (102) |
| 2A | 60.0 ± 3.8 (95) | 61.0 ± 2.1 (97) |
| 2B | 65.0 ± 3.9 (103) | 62.0 ± 4.8 (98) |
| 3A | 64.7 ± 4.0 (103) | 65.0 ± 2.9 (103) |
| 3B | 60.7 ± 4.4 (96) | 59.7 ± 4.7 (95) |
| 4A | 64.7 ± 5.7 (103) | 64.3 ± 3.0 (102) |
| 4B | 67.3 ± 5.0 (107) | 61.3 ± 5.0 (97) |
| 5A | 65.3 ± 4.5 (104) | 66.0 ± 4.8 (105) |
| 5B | 62.7 ± 2.9 (100) | 59.3 ± 4.0 (94) |
Notes:
a
Concentrations investigated for all compounds were 10 and 20 μM except for compounds 1B and 5B – the concentrations used were 5 and 10 μM. No significant changes in the viability of Salmonella typhimurium TA1535 treated with bichalcophenes, B[a]P, and S9 mix when compared to non-treated bacteria. Assays were performed in triplicates with a 10−7 dilution of an overnight bacteria.