Table 5.
Determination of mutagenic activity of bichalcophenes in Salmonella typhimurium TA1535 in presence of S9 mix
| Compounda |
Bichalcophene mutagenicity (revertant colonies/plate; mean ± SEM)
|
|
|---|---|---|
| 5/10 μM | 10/20 μM | |
| None (spontaneous) | 27.7 ± 2.3 | |
| B[a]P (20 μM) | 347.7 ± 33.9b | |
| 1A | 13.3 ± 1.5 | 23.7 ± 2.5 |
| 1B | 14.0 ± 2.0 | 11.3 ± 1.0 |
| 2A | 29.7 ± 3.2 | 23.7 ± 2.5 |
| 2B | 14.3 ± 2.1 | 16.7 ± 1.9 |
| 3A | 15.3 ± 4.2 | 24.7 ± 2.5 |
| 3B | 11.7 ± 1.5 | 13.7 ± 1.5 |
| 4A | 28.7 ± 3.1 | 34.0 ± 3.0 |
| 4B | 30.3 ± 3.5 | 24.0 ± 2.7 |
| 5A | 15.3 ± 1.5 | 26.7 ± 2.1 |
| 5B | 15.7 ± 2.0 | 13.0 ± 2.0 |
Notes:
a
Concentrations investigated for all compounds were 10 and 20 μM except for compounds 1B and 5B, the concentrations used were 5 and 10 μM;
b
significantly different (P < 0.05) from non-treated bacteria (spontaneous mutations). No mutagenic activity of bichalcophenes/S9 mix recorded in Salmonella typhimurium TA1535 when compared to non-treated or B[a]P-treated bacteria. Assays were performed in triplicate.
Abbreviation: SEM, standard error of the mean.