. 2013 Feb 4;7:73–81. doi: 10.2147/DDDT.S40129

Table 6.

Determination of anti-mutagenic activity of bichalcophenes in Salmonella typhimurium TA1535 against sodium azide (NaN₃; 1 μg/plate)

Compound^a	NaN₃ mutagenicity at bichalcophene concentrations (revertant colonies/plate; mean ± SEM (% reduction in NaN₃ mutagenicity))
	Pre-exposure		Co-exposure
	5/10 μM	10/20 μM	5/10 μM	10/20 μM
NaN₃	384.7 ± 14.7 (0)
1A	47.0 ± 3.5 (96)^b	48.3 ± 6.8 (96)^b	59.7 ± 9.0 (93)^b	28.3 ± 3.1 (102)^b,c
1B	56.7 ± 8.6 (94)^b	61.7 ± 11.4 (92)^b	35.0 ± 3.0 (100)^b	27.3 ± 9.3 (102)^b
2A	53.0 ± 9.8 (95)^b	70.7 ± 12.9 (90)^b	39.3 ± 7.6 (99)^b	47.3 ± 6.0 (96)^b
2B	43.7 ± 10.4 (97)^b	51.0 ± 7.8 (95)^b	63.0 ± 7.6 (92)^b	19.3 ± 1.5 (104)^b,c
3A	47.7 ± 3.5 (96)^b	52.0 ± 5.0 (95)^b	95.0 ± 4.0 (83)^b	42.3 ± 9.1 (98)^b,c
3B	37.3 ± 2.1 (99)^b	33.7 ± 3.5 (100)^b,d	26.7 ± 6.7 (102)^b,d	17.3 ± 2.1 (105)^b
4A	186.3 ± 14.2 (57)^b	103.0 ± 9.2 (80)^b,c	32.3 ± 4.2 (101)^b	39.7 ± 6.0 (98)^b
4B	35.0 ± 4.0 (100)^b,d	35.0 ± 4.5 (100)^b,d	80.3 ± 6.1 (87)^b,d	73.3 ± 7.9 (89)^b,d
5A	61.3 ± 8.1 (92)^b	97.7 ± 10.2 (82)^b,c	47.0 ± 3.5 (96)^b	32.3 ± 2.5 (101)^b
5B	35.7 ± 3.1 (100)^b	37.0 ± 5.3 (99)^b,d	21.3 ± 6.4 (104)^b,d	15.3 ± 3.2 (105)^b

Notes:

Concentrations investigated for all compounds were 10 and 20 μM except for compounds 1B and 5B – the concentrations used were 5 and 10 μM. Assays were performed in triplicate. The spontaneous revertant colonies were 34.3 ± 4.0;

significant (P < 0.05) reduction (% of inhibition of mutagenicity indicated in parentheses) from revertant colonies seen with NaN₃, 40% or more reduction means strong antimutagenic activity;

significant difference (P < 0.05) between bichalcophene concentrations;

significant difference (P < 0.05) between mononitril (A compounds) versus corresponding monocationic (B compounds).

Abbreviation: SEM, standard error of the mean.