Table 7.
Determination of anti-mutagenic activity of bichalcophenes in Salmonella typhimurium TA1535 against benzo[a]pyrene (B[a]P; 20 μM) in presence of S9 mix
| Compounda |
B[a]P mutagenicity at bichalcophene concentrations (revertant colonies/plate; mean ± SEM (% reduction in B[a]P mutagenicity))
|
|||
|---|---|---|---|---|
|
Pre-exposure
|
Co-exposure
|
|||
| 5/10 μM | 10/20 μM | 5/10 μM | 10/20 μM | |
| B[a]P | 290.3 ± 21.0 (0) | |||
| 1A | 270.0 ± 25.9 (9) | 239.3 ± 22.8 (24)b | 274.0 ± 25.2 (8) | 240.3 ± 27.7 (23) |
| 1B | 194.7 ± 16.6 (44)b | 153.3 ± 5.9 (64)b,c | 207.3 ± 17.9 (38)b | 174.3 ± 21.4 (54)b |
| 2A | 112.7 ± 21.1 (82)b | 120.0 ± 17.4 (79)b | 261.0 ± 42.7 (14) | 264.0 ± 30.4 (12) |
| 2B | 101.7 ± 12.1 (87)b | 100.0 ± 8.2 (88)b | 256.3 ± 14.2 (16) | 230.7 ± 17.6 (28) |
| 3A | 264.0 ± 6.1 (12) | 250.0 ± 16.5 (19) | 267.0 ± 21.5 (11) | 255.7 ± 27.0 (16) |
| 3B | 228.0 ± 25.2 (29)b | 227.7 ± 16.3 (29)b | 239.7 ± 29.5 (23) | 216.3 ± 16.5 (34)b |
| 4A | 148.7 ± 16.3 (66)b | 120.3 ± 10.1 (79)b | 254.7 ± 19.6 (17) | 224.7 ± 30.7 (30) |
| 4B | 119.7 ± 12.7 (79)b | 108.0 ± 9.5 (85)b | 249.7 ± 35.4 (19) | 231.7 ± 23.7 (27) |
| 5A | 256.7 ± 14.6 (16) | 256.0 ± 15.7 (16) | 257.7 ± 16.0 (15) | 255.7 ± 13.4 (16) |
| 5B | 109.0 ± 10.8 (84)b,c | 95.7 ± 14.1 (90)b,c | 171.7 ± 27.2 (55)b,c | 119.3 ± 11.6 (79)b,c |
Notes:
Concentrations investigated for all compounds were 10 and 20 μM except for compounds 1B and 5B – the concentrations used were 5 and 10 μM. Assays were performed in triplicate. The spontaneous revertant colonies were 74.7 ± 5.5;
significant (P < 0.05) reduction (% of inhibition of mutagenicity indicated in parentheses) from revertant colonies seen with B[a]P, 20% or less means no activity, 20%–40% indicates moderate activity, and 40% or more reduction means strong antimutagenic activity;
significant difference (P < 0.05) between mononitril (A compounds) versus corresponding monocationic (B compounds).
Abbreviation: SEM, standard error of the mean.