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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: FEBS J. 2012 Oct 30;279(23):4283–4292. doi: 10.1111/febs.12018

Figure 2.

Figure 2

Cleavage of the Leu81-Glu82 peptide bond in human cationic trypsinogen by human pancreatic chymotrypsins and elastases. Trypsinogen (2 μM) was incubated with 25 nM of the indicated proteases in 0.1 M Tris-HCl (pH 8.0) in the presence of 20 nM SPINK1 at 37 °C (final concentrations). (A) At the indicated times, aliquots were withdrawn, precipitated with trichloroacetic acid and analyzed by reducing SDS-PAGE and Coomassie blue staining, as described in Experimental Procedures. Representative gels of two or three experiments are shown. (B) The decrease in the intensity of the trypsinogen band was quantitated by densitometry. Error bars were omitted for clarity, the error was within 10% of the mean.