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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Mol Biol. 2011 Mar 17;408(4):755–765. doi: 10.1016/j.jmb.2011.03.015

Fig. 9.

Fig. 9

Structure–dynamics function relationships for AFA-PLN, Ser16 phosphorylated AFA-PLN, and pseudo-phosphorylated mutants. (a) Correlation between combined 15N-HSQC chemical shift change (normalized Δδ), averaged [1H,15N] NOE in domain Ia and the loop (b), and normalized solvent exchange from SEA-CLEANEX experiments (c) with SERCA inhibition from ATPase assays (ΔpKCa) for AFA-PLN (black), S16E-AFA-PLN (blue), S16D-AFA-PLN (gray), and pS16-AFA-PLN (red). See Materials and Methods for calculation of ΔpKCa, normalized Δδ, 〈[1H,15N] NOE〉, and normalized solvent exchange.