Structure–dynamics function relationships for AFA-PLN, Ser16 phosphorylated AFA-PLN, and pseudo-phosphorylated mutants. (a) Correlation between combined 15N-HSQC chemical shift change (normalized Δδ), averaged [1H,15N] NOE in domain Ia and the loop (b), and normalized solvent exchange from SEA-CLEANEX experiments (c) with SERCA inhibition from ATPase assays (ΔpKCa) for AFA-PLN (black), S16E-AFA-PLN (blue), S16D-AFA-PLN (gray), and pS16-AFA-PLN (red). See Materials and Methods for calculation of ΔpKCa, normalized Δδ, 〈[1H,15N] NOE〉, and normalized solvent exchange.