Table 1.

Virus production and infectivity.

Virus	Virus Production (%)a	Infectivity (%)b
		HIV-1 env+	VSV-G Pseudotyped HIV-1c
WT	100	100	100
Y145A	63 ± 13d	≤0.5	≤10
S146A	81 ± 8	61 ± 11	105 ± 18
P147L	65 ± 19	4 ± 2	86 ± 32
T148A	77 ± 14	86 ± 11	110 ± 18
S149A	65 ± 24	5 ± 1	102 ± 32
I150A	59 ± 12	≤0.5	≤10
L151A	50 ± 11	≤0.5	≤10

^aVirus production was assayed as RT activity in the supernatant of transfected cells. Values were converted to percentage of WT level for each of three or more independent transfections and were averaged.

^bInfectivity was measured in the single-round LuSIV assay (Roos et al., 2000). WT infectivity was set at 100%.

^cHIV-1 env ⁻ virions were pseudotyped with VSV-G envelope protein, as described in Materials and methods.

^dNumbers to the right of the “±” represent the standard deviation.