Table 2.
Recovery of phytohormones from different sample matrices on polystyrene/ divinylbenzene-based Cromabond® HR-XC material
| Matrix | SA | ABA | JA | 12-OH-JA | JA-Ile | OPDA | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
Slope |
R2 |
Rec. |
Slope |
R2 |
Rec. |
Slope |
R2 |
Rec. |
Slope |
R2 |
Rec. |
Slope |
R2 |
Rec. |
Slope |
R2 |
Rec. |
|
Recovery control |
453.48 |
0.99 |
1.00 |
75.12 |
0.99 |
1.00 |
105.27 |
0.99 |
1.00 |
431.91 |
0.99 |
1.00 |
541.36 |
0.99 |
1.00 |
126.64 |
0.99 |
1.00 |
|
20% Methanol |
397.59 |
0.99 |
0.88 |
90.73 |
0.99 |
1.21 |
110.37 |
0.97 |
1.05 |
497.68 |
1.00 |
1.15 |
533.24 |
1.00 |
0.98 |
122.33 |
1.00 |
0.97 |
|
Methanolic extract |
558.56 |
0.96 |
1.23 |
76.50 |
0.92 |
1.02 |
108.49 |
0.97 |
1.03 |
444.99 |
0.98 |
1.03 |
532.82 |
1.00 |
0.98 |
118.41 |
0.99 |
0.93 |
| Reconstituted methanolic extract | 392.81 | 0.77 | 0.86 | 55.25 | 0.86 | 0.49 | 81.27 | 0.88 | 0.64 | 156.24 | 0.83 | 0.44 | 424.25 | 0.99 | 0.67 | 77.65 | 0.95 | 0.50 |
The analyte abundances (peak heights) were plotted against their defined concentrations and correlation of these two parameters was estimated (linear regression coefficient was calculated). The phytohormone recoveries (Rec.) were determined as ratios of slopes compairing corresponding concentration-intensity plots obtained with and without SPE procedure.