Table 1.
A partial list of Filamin-A interacting partners
| Interacting partners | Binding sites* | Approach** | Significance | Reference |
|---|---|---|---|---|
|
Cytoskeleton and cell shape maintenance | ||||
| F-actin |
ABD, Rod-1 |
b |
3D F-actin networks with unique mechanical and physiological properties |
[47,54] |
| Calmodulin |
ABD |
b |
Regulates F-actin binding in vitro |
[55] |
| R-Ras |
3 |
b, c |
Enhances integrin activation and maintains endothelial barrier |
[56,57] |
| Syk |
5 |
b, c |
Supports ITAM-mediated receptor signaling in platelet |
[58] |
| Vimentin |
1-8 |
c |
Vimentin phosphorylation, cell surface expression of β1 integrins and cell spreading on collagen |
[52,53] |
| Supervillin |
8–10, 20–22 |
a |
Cell spreading |
[59] |
|
Membrane and membrane associated proteins | ||||
| Dopamine D2 and D3 receptors |
19 |
a, b, c |
Stabilizes β-arrestins-filamin-A complex |
[60,61] |
| Pro-Prion |
10,16–18, 20, 21, 23 |
b, c |
Enhances the binding of filamin-A with β1 integrin, and promotes cell spreading and migration in melanoma |
[62,63] |
| GPI bα (CD 42b) |
17 |
b, c |
Intracellular trafficking and maintains the size of platelets |
[64,65] |
| β Integrins |
21 |
c |
Adhesion, mechanoprotection and competing binding site with talin to regulate integrin activation |
[66,67] |
| Tissue factor |
22-24 |
a, b |
Supports cell spreading and migration |
[68] |
| CEACAM 1 |
23–24 |
a, b |
Reduces cell migration |
[69] |
| Migfilin (FBLP-1) |
21 |
a, b, c |
Disconnects filamin-A from integrin and promotes talin-integrin binding |
[48,70-72] |
| Caveolin-1 |
22-24 |
a, b |
Intracellular trafficking |
[73,74] |
|
Intracellular signaling | ||||
| β-arrestins |
22 |
a, b, c |
ERK activation and actin cytoskeleton reorganization |
[75] |
| Wee1 |
22-24 |
b, c |
Regulates Wee1 expression and promotes G2/M phase progression |
[42] |
| K-RAS |
nd |
nd |
Filamin-A deficiency reduces K-RAS oncogenic potentials |
[76] |
| NIK |
nd |
b |
Mediates the activation of the IKKα/NF-κB cascade through CD28 signaling |
[77] |
| sst2 |
19-20, 21-24 |
b, c |
Negative control on PI3K pathway |
[78] |
| Androgen receptor |
16-19 |
a, b, c |
Required for androgen-induced cell migration |
[79,80] |
| SEK1 |
22-23 |
a, b, c |
Tumor necrosis factor-alpha signaling |
[81] |
| TRAF2 |
15-19 |
a, b, c |
Inflammatory signal transduction |
[82] |
|
Small GTP-binding proteins and their regulators | ||||
| Rho/Cdc42/RalA |
24 |
c |
Remodeling of cytoskeleton |
[83] |
| ROCK |
24 |
b, c |
Remodeling of cytoskeleton |
[84] |
| FilGAP |
23 |
a, b, c |
Cell spreading and GAP activation |
[17,85] |
| Trio |
23–24 |
c |
GEF for RhoG/Rac1 and RhoA and required for ruffling |
[86] |
|
Nuclear function associated proteins | ||||
| BRCA1 |
23-24 |
a, b, c |
Facilitates the recruitment of BRAC1 and RAD51 to DNA damage sites and stabilizes the DNA-PK holoenzyme |
[87] |
| BRCA2 |
21-24 |
a, b, c |
Required for efficient homologous recombination DNA repair and recovery of G2/M phase arrest |
[88-90] |
| RefilinB |
15-24 |
a, b |
Stabilizes perinuclear actin actin networks and regulates nuclear shape |
[91] |
| TIF-IA, RPA40 |
ABD |
b |
Suppresses ribosomal RNA gene transcription |
[92] |
| TAF1B/mKIAA1093 | 1-7 | a, c | Possible role in rRNA production, protein translation and the organization of centromeres | [93] |
Note:
*: This column indicates the domains involved in the respective interactions. Numbers in this column represent the repeat numbers of filamin-A.
**: The approaches used to demonstrate the interaction including, (a) yeast two-hybrid; (b) in vitro pull-down; (c) co-immunoprecipitation; or (nd), not determined.