Table 1.
Sensitivities of primary neurons, SH-SY5Y human neuroblastomas and ESN to the seven classical BoNT serotypes
| BoNT serotype | Target SNARE protein | Primary neurons EC50(pM) | SH-SY5Y EC50(pM) | ESNs EC50(pM) |
95% confidence intervals |
|
|---|---|---|---|---|---|---|
| - | + | |||||
| BoNT/A |
SNAP-25 |
0.4A |
5562 |
0.80 |
0.74 |
0.86 |
| 10B | ||||||
| BoNT/B |
VAMP2 |
100B |
41,650 |
22.50 |
12.91 |
39.30 |
| BoNT/C |
SNAP-25 |
13A |
nr |
14.24 |
9.68 |
20.93 |
| Syntaxin-1 |
nr |
nr |
7.51 |
3.06 |
18.42 |
|
| BoNT/D |
VAMP2 |
nr |
2560 |
3.09 |
2.49 |
3.83 |
| BoNT/E |
SNAP-25 |
36A |
nr |
66.73 |
52.12 |
85.44 |
| 43B | ||||||
| BoNT/F |
VAMP2 |
1350B |
≫300,000 |
157.82 |
133.25 |
186.92 |
| BoNT/G | VAMP2 | nr | nr | 689.76 | 615.90 | 772.48 |
Notes: Primary neurons’ values are compiled from two sources. Annotation by (A) represents 50% inhibition of neurotransmitter release in rat cerebellar neuron cultures exposed to BoNTs for 24 h (a 90% correlation was shown between neurotransmitter release and SNARE integrity) [29], whereas annotation by (B) represents cleavage of 50% of SNAP-25 protein in fetal mouse spinal cord cultures exposed to BoNT for 48 h [30]. SH-SY5Y neuroblastomas’ values indicate inhibition of 50% of noradrenalin release in SH-SY5Y human neuroblastomas cells after 72 h incubation [2]. ESN values represent 50% cleavage of SNARE proteins after a 24 h tonic intoxication. ESN EC50 values for BoNT/B, /C, /D, /F and /G are from this manuscript, while BoNT/A and /E are from McNutt et al. (2011). nr, not reported.