Table 3.

List of primers used for PCR amplification of protein-encoding genes from Treponema denticola strains

Gene	Primer	Sequence(5′to 3′)	Strains amplified
dnaN	dnaNF	ATGAAAATAAGTTTTGACAGAGACAC	dnaF + dnaR: all strains (55-50°C)
	dnaNR	TTACTCCGTCTGCATAGGC
recA	recAF1	GTGGCAAAAGCAAAAAAC	recAF1 + recAR1: most strains (55-47°C)
	recAR1	TTAAAAAAGACTGTCGTCCG	recAF2 + recAR2: ATCC 700768, MS25 (54-47°C)
	recAF2	TTCATATTGGCCGCATTTG	recAF1 + recArecAR2: ATCC 700771 (55-49°C)
	recAR2	TTGTGTACTCATAATGCCGCTC
	recAF	GTGGCAAAAGCAAAAAACGAAG	recAF + recAR: OMZ852, OMZ853, NY531, NY553 (58-53°C)
	recAR	TTAAAAAAGACTGTCGTCCGCC
radC	radCF1	ATGATAGACTATAAAAATTCGTCCAATAC	radCF1 + radCR1: most strains (55-50°C)
	radCR1	TTAAATATCAAACCTCGTTCCG	radCF1 + radCR2: MS25 (55-49°C)
	radCF2	AACATGGCTTTCCGAAATC	radCF2 + radCR1: ATCC 700768 (55-49°C)
	radCR2	GTGCAGCGGCTCTAAAAG
ppnK	TDE1591F1	ATATGGATCCCATATGAAAAAAG	TDE1591F1 + TDE1591R1: most strains (52-45°C)
	TDE1591R1	AATTCTCGAGTCAATTCAGTTTGGG	TDE1591F2 + TDE1591R2: OKA3, MS25,GM1, ST10A,
	TDE1591F2	AGCTACCCTGCCCTAATTTC	ATCC 700768, ATCC 700771 (57-52°C)
	TDE1591R2	AACATCCTTAAAAAGCGGC
flaA	TDE1712F	ATGAAAAAAACATTTATACTTGTTG	TDE1712F + TDE1712R: all strains (52-46°C)
	TDE1712R	TTATTGTTGGTTCTTTTCGG
era	eraF1	ATGAACAGCGGAGTTGTAAC	eraF1 + eraR1: most strains (55-50°C)
	eraR1	TTAATACGAGATTTTTTTTATGATATTATC
	eraF2	GGTACTTGTGCTTACCGAAAAC	eraF2 + eraR2: MS25 (54-47°C)
	eraR2	CCGACACAATCGAGGAAG
	eraF4	CGCTTAGAAGAAGGGGATGC	eraF4, eraR4 separately used for direct chromosome sequencing of ATCC 700768†
	eraR4	CTTTTTCGACATAGAGGAAGGC
pyrH	pyrHF	ATGGTAACTGTTTTGTCGGT	pyrHF + pyrHR: all strains (54-47°C)
	pyrHR	TTAGCCGATTACCGTTCCTT

Genetic loci are based on the ATCC 35405 type strain of Treponema denticola.

F: Forward primer; R: Reverse primer. Values in parenthesis indicate annealing temperatures used in ‘touchdown PCR’ procedures.

^†PCR amplification was unsuccessful; sequencing of chromosomal DNA employed.