Table 1. Genotyping Assays of MicroRNA SNPs.
MicroRNA | SNP (Base Change) | Primers Used (Sense/Antisense) | PCR Product | Enzyme | Digested Products |
miR146 rs2910164 | G>C | 5′-AGGAGGGGTCTTTGC ACCATC-3′ 5′-CCCAGCTGA AGA ACTGAA CTGCA -3′ | 113 bp | PstI | G allele: 113 bp C allele: 89 bp/24 bp |
miR149 rs2292832 | C>T | 5′-ATGTCCAGGACCACA ACCTGT-3′ 5′-CACCTCTCACACCCCCTCAC-3′ | 337 bp | PvuII | C allele: 337 bp T allele: 220 bp/117 bp |
miR196 rs11614913 | C>T | 5′-CAGCTGATCTGTGGCTTAGGT-3′ 5′-GAAAACCGACTGATGTAACCCAG-3 | 93 bp | MvaI | T allele: 93 bp C allele: 70 bp/23 bp |
miR499 rs3746444 | C>T | 5′-ACGGGAAGCAGCACAGACTTG-3′ 5′-TGTTTAACTCCTCTCCACGTGTAC-3′ | 52 bp | BsrGI | C allele: 52 bp T allele: 27 bp/25 bp |