Figure 1. Conditioned medium derived from RV-infected HBEC had increased concentrations of protein and IL-6; and caused a decrease in isoprenaline induced cAMP from ASMCs without an effect on ASMC viability.

(A–B) HBEC (n = 4) were uninfected (Control) or exposed to: UV inactivated RV (UVi-RV) or replication competent RV (RV) at an MOI = 2 for 24 hours. The concentration of total protein and IL-6 in the supernatant was measured using a BCA assay and ELISA respectively. (C–D) ASMCs (n = 14) were treated with conditioned medium from HBEC (n = 3) that were uninfected, (Control) or exposed to: UV inactivated RV (UVi-RV) or replication competent RV (RV) at an MOI = 2 for 3 days. Isoprenaline induced cAMP was measured using a cAMP functional assay and ASMC viability was measured using a MTT assay. Data represent mean ± SEM. Statistical differences were examined for using 1-way ANOVA with Bonferroni post test comparison to control treatment *p<0.05.