Figure 1. Increased Cyr61 expression in the kidneys after unilateral ureteral obstruction (UUO) surgery.

(A–D) The Q-PCR time-course for transcripts of whole kidney Cyr61 (A), monocyte chemoattractant protein-1 (MCP-1) (B), connective tissue growth factor (CTGF) (C), and collagen type I -α1 (Co l 1-α1) (D) following UUO surgery. The expression levels were normalized by 18S ribosomal RNA. N = 8/time point. (E) Representative images of the time-course Western blot of whole kidney lysates for Cyr61 after surgery. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (F) Bar chart showing the Cyr61 protein expression time-course normalized by GAPDH. N = 8/time point. The values are the mean+SD. *P<0.05 vs. sham operation; #P<0.05 vs. contralateral (CLK) kidney.